Imaging:Article Title: Effects of curcumin on hippocampal expression of NgR and axonal regeneration in Aβ-induced cognitive disorder rats.
Article Snippet: .. The main experimental reagents and apparatus were as follows: Aβ1-40 peptides 2041 ©FUNPEC-RP www.funpecrp.com.brGenetics and Molecular Research 13 (1): 2039-2047 (2014) Effects of curcumin on NgR and axonal regeneration (Sigma, USA), curcumin (Sigma), goat anti-NgR antibody and mouse anti-Neurofilament 200 kDa antibody (dilution of 1:100 and 1:200, respectively; Wuhan Boster Biological Technology, China), Morris water maze and brain stereotaxic instrument (Narishige, Japan), gel imaging system (Bio-Rad, USA), BS-50 optical microscopy (Olympus, German), CM2000B image analysis system (Beijing University of Aeronautics and Astronautics, China), reverse transcription-polymerase chain reaction (RT-PCR) kits (Takara Biotechnology, Co., Ltd., Dalian, China), NgR primers (upstream sequence: 5'-TGC TGG CAT GGG TGT TAT GG-3', downstream sequence: 5'-CGG AAG GTG TTG TCG GGA AG-3', length of amplified fragment: 493 bp), β-actin (upstream sequence: 5'-CGT AAA GAC CTC TAT GCC AAC A-3', downstream sequence: 5'-CGG ACT CAT CGT ACT CCT GCT-3', length of amplified fragment: 229 bp). .. Fifty-three healthy male rats (230-250 g) were provided by the Experimental Animal Center of Chongqing Medical University, China.
Microscopy:Article Title: Effects of curcumin on hippocampal expression of NgR and axonal regeneration in Aβ-induced cognitive disorder rats.
Article Snippet: .. The main experimental reagents and apparatus were as follows: Aβ1-40 peptides 2041 ©FUNPEC-RP www.funpecrp.com.brGenetics and Molecular Research 13 (1): 2039-2047 (2014) Effects of curcumin on NgR and axonal regeneration (Sigma, USA), curcumin (Sigma), goat anti-NgR antibody and mouse anti-Neurofilament 200 kDa antibody (dilution of 1:100 and 1:200, respectively; Wuhan Boster Biological Technology, China), Morris water maze and brain stereotaxic instrument (Narishige, Japan), gel imaging system (Bio-Rad, USA), BS-50 optical microscopy (Olympus, German), CM2000B image analysis system (Beijing University of Aeronautics and Astronautics, China), reverse transcription-polymerase chain reaction (RT-PCR) kits (Takara Biotechnology, Co., Ltd., Dalian, China), NgR primers (upstream sequence: 5'-TGC TGG CAT GGG TGT TAT GG-3', downstream sequence: 5'-CGG AAG GTG TTG TCG GGA AG-3', length of amplified fragment: 493 bp), β-actin (upstream sequence: 5'-CGT AAA GAC CTC TAT GCC AAC A-3', downstream sequence: 5'-CGG ACT CAT CGT ACT CCT GCT-3', length of amplified fragment: 229 bp). .. Fifty-three healthy male rats (230-250 g) were provided by the Experimental Animal Center of Chongqing Medical University, China.
Reverse Transcription Polymerase Chain Reaction:Article Title: Effects of curcumin on hippocampal expression of NgR and axonal regeneration in Aβ-induced cognitive disorder rats.
Article Snippet: .. The main experimental reagents and apparatus were as follows: Aβ1-40 peptides 2041 ©FUNPEC-RP www.funpecrp.com.brGenetics and Molecular Research 13 (1): 2039-2047 (2014) Effects of curcumin on NgR and axonal regeneration (Sigma, USA), curcumin (Sigma), goat anti-NgR antibody and mouse anti-Neurofilament 200 kDa antibody (dilution of 1:100 and 1:200, respectively; Wuhan Boster Biological Technology, China), Morris water maze and brain stereotaxic instrument (Narishige, Japan), gel imaging system (Bio-Rad, USA), BS-50 optical microscopy (Olympus, German), CM2000B image analysis system (Beijing University of Aeronautics and Astronautics, China), reverse transcription-polymerase chain reaction (RT-PCR) kits (Takara Biotechnology, Co., Ltd., Dalian, China), NgR primers (upstream sequence: 5'-TGC TGG CAT GGG TGT TAT GG-3', downstream sequence: 5'-CGG AAG GTG TTG TCG GGA AG-3', length of amplified fragment: 493 bp), β-actin (upstream sequence: 5'-CGT AAA GAC CTC TAT GCC AAC A-3', downstream sequence: 5'-CGG ACT CAT CGT ACT CCT GCT-3', length of amplified fragment: 229 bp). .. Fifty-three healthy male rats (230-250 g) were provided by the Experimental Animal Center of Chongqing Medical University, China.
Sequencing:Article Title: Effects of curcumin on hippocampal expression of NgR and axonal regeneration in Aβ-induced cognitive disorder rats.
Article Snippet: .. The main experimental reagents and apparatus were as follows: Aβ1-40 peptides 2041 ©FUNPEC-RP www.funpecrp.com.brGenetics and Molecular Research 13 (1): 2039-2047 (2014) Effects of curcumin on NgR and axonal regeneration (Sigma, USA), curcumin (Sigma), goat anti-NgR antibody and mouse anti-Neurofilament 200 kDa antibody (dilution of 1:100 and 1:200, respectively; Wuhan Boster Biological Technology, China), Morris water maze and brain stereotaxic instrument (Narishige, Japan), gel imaging system (Bio-Rad, USA), BS-50 optical microscopy (Olympus, German), CM2000B image analysis system (Beijing University of Aeronautics and Astronautics, China), reverse transcription-polymerase chain reaction (RT-PCR) kits (Takara Biotechnology, Co., Ltd., Dalian, China), NgR primers (upstream sequence: 5'-TGC TGG CAT GGG TGT TAT GG-3', downstream sequence: 5'-CGG AAG GTG TTG TCG GGA AG-3', length of amplified fragment: 493 bp), β-actin (upstream sequence: 5'-CGT AAA GAC CTC TAT GCC AAC A-3', downstream sequence: 5'-CGG ACT CAT CGT ACT CCT GCT-3', length of amplified fragment: 229 bp). .. Fifty-three healthy male rats (230-250 g) were provided by the Experimental Animal Center of Chongqing Medical University, China.
Amplification:Article Title: Effects of curcumin on hippocampal expression of NgR and axonal regeneration in Aβ-induced cognitive disorder rats.
Article Snippet: .. The main experimental reagents and apparatus were as follows: Aβ1-40 peptides 2041 ©FUNPEC-RP www.funpecrp.com.brGenetics and Molecular Research 13 (1): 2039-2047 (2014) Effects of curcumin on NgR and axonal regeneration (Sigma, USA), curcumin (Sigma), goat anti-NgR antibody and mouse anti-Neurofilament 200 kDa antibody (dilution of 1:100 and 1:200, respectively; Wuhan Boster Biological Technology, China), Morris water maze and brain stereotaxic instrument (Narishige, Japan), gel imaging system (Bio-Rad, USA), BS-50 optical microscopy (Olympus, German), CM2000B image analysis system (Beijing University of Aeronautics and Astronautics, China), reverse transcription-polymerase chain reaction (RT-PCR) kits (Takara Biotechnology, Co., Ltd., Dalian, China), NgR primers (upstream sequence: 5'-TGC TGG CAT GGG TGT TAT GG-3', downstream sequence: 5'-CGG AAG GTG TTG TCG GGA AG-3', length of amplified fragment: 493 bp), β-actin (upstream sequence: 5'-CGT AAA GAC CTC TAT GCC AAC A-3', downstream sequence: 5'-CGG ACT CAT CGT ACT CCT GCT-3', length of amplified fragment: 229 bp). .. Fifty-three healthy male rats (230-250 g) were provided by the Experimental Animal Center of Chongqing Medical University, China.
Incubation:Article Title: Nogo enhances the adhesion of olfactory ensheathing cells and inhibits their migration.
Article Snippet: .. Briefly, coverslips were fixed with 4% paraformaldehyde in PBS for 20 minutes, then permeabilized with or without 0.3% Triton X-100 in 0.1 M PBS for 15 minutes and incubated overnight at 4°C with polyclonal antibodies to NgR (Santa Cruz), S-100 (Boster) or Nogo-A (Santa Cruz), or monoclonal antibodies to paxillin (BD Transduction Laboratories), O1 (R&D Systems) or MBP (myelin basic protein, Chemicon international) diluted in PBS containing 10% normal goat serum. .. After washing three times with 0.1 M PBS (pH 7.4), cells were incubated with fluorescence-conjugated secondary antibody (Sigma) for 90 minutes at room temperature.
Bioprocessing:Article Title: Nogo enhances the adhesion of olfactory ensheathing cells and inhibits their migration.
Article Snippet: .. Briefly, coverslips were fixed with 4% paraformaldehyde in PBS for 20 minutes, then permeabilized with or without 0.3% Triton X-100 in 0.1 M PBS for 15 minutes and incubated overnight at 4°C with polyclonal antibodies to NgR (Santa Cruz), S-100 (Boster) or Nogo-A (Santa Cruz), or monoclonal antibodies to paxillin (BD Transduction Laboratories), O1 (R&D Systems) or MBP (myelin basic protein, Chemicon international) diluted in PBS containing 10% normal goat serum. .. After washing three times with 0.1 M PBS (pH 7.4), cells were incubated with fluorescence-conjugated secondary antibody (Sigma) for 90 minutes at room temperature.
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